ABSTRACT
The phytochemical investigation of Vitex pinnata led to the isolation of a mixture of steroids β-sitosterol and stigmasterol (1a and 1b) and three known flavonoid identified as 5-hydroxy-3, 7, 4’-trimethoxyflavone (2), 5-hydroxy-7,4’-dimethoxy-flavone (3) and 5-hydroxy-3,3’,4’,7-tetramethoxyflavone (4). The structures of all isolated compounds were carried out by NMR and mass spectrometry. The isolated compounds were evaluated for their anti-infective activities against Trypanosoma brucei brucei and Mycobacterium marinum. Compound 1-4 showed moderate antitrypanosomal activity with MIC values of 6.25μg/ml, 19.0, 21.0 and 17.0μM, respectively while no activity observed on anti-mycobacterial. This study is the first to report the presence of three flavones and their antitrypanosomal activity from V. pinnata.
ABSTRACT
Chromatographic procedures from the aerial parts of Abies nobilis and stem barks of Prosopis africana led to the isolation of two (antimicrobial and cytotoxic) compounds. Their structures were established as 7, 3’, 4’- trihydroxy-3-methoxyflavanone and dehydroabietic acid on the basis of spectroscopic techniques.